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Interacting residues and surfaces were determined using the protein interfaces, surfaces and assemblies service PISA at the European Bioinformatics Institute 63 ( and ChimeraX 64

At the systemic level, 1,25-(OH) 2 D 3 enhances intestinal calcium absorption by binding to vitamin D receptor and inducing the expression of the calcium-binding protein Calbindin-D9k

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For the DEN-induced mouse model, DEN at 25 mg/kg was injected into 2-week-old C57BL/6 mice intraperitoneally to induce spontaneous HCC formation